Green Synthesis of Silver Nanoparticles Using Sphaeranthus Indicus Leaf Extract and Their Antibacterial Activity

The present work deals with Sphaeranthus indicus leaf extract used as a capping and reducing agent for silver nanoparticles (Ag NPs) synthesis. Synthesized nanoparticles were characterized by UV-Vis, FT-IR, XRD, SEM with EDX and TEM analysis. The UV–Visible spectra of Ag NPs exhibited a surface plasmon resonance peak at 424 nm. FT-IR analysis determined the vibration functional group to responsible for formation of Ag NPs. XRD patterns revealed that face-center cubic phase structure. SEM images clearly showed spherical nature of the Ag NPs. The EDX analysis showed the complete elemental composition of the synthesized Ag NPs. TEM images of Ag NPs revealed the spherical shapes with an average particle size of 20 nm. Synthesized Ag NPs was investigated against Gram positive and negative bacterial strains were tested with different concentrations of 10, 30, 50, 70 and 100 L. The zone of inhibition varied significantly in a dose-dependent manner. In future, it can be used for against human pathogenic bacterial strains as well as to photo-catalytic, textile fabric and co-formulate detergents.